Isolation of Individual Compounds from the Aerial Part of <i>Comarum palustre </i>L. and Their Structure Elucidation Using Spectroscopic Methods

Introduction. Medicinal plants are a rich, almost inexhaustible source of medicinal substances, and due to their large chemical diversity metabolites available for isolation research is always an important task. One the promising marsh cinquefoil ( Comarum palustre L.), widely used in folk medicine treatment diseases musculoskeletal system. Aim. Isolation individual secondary from aerial part C. subsequent structural elucidation by NMR experiments. Materials methods. The parts cinquefoil, were collected next Saint Petersburg State Chemical-Pharmaceutical University Nursery Garden Plants (Leningrad region, Vsevolozhsky district, Priozerskoe highway, 38 km) July 2021. Fraction analysis was performed through analytical high-performance liquid chromatography (HPLC) using LC-20 Prominence (Shimadzu corp., Japan) equipped with SPD-M20A diode-array detector. compounds carried out open column sorbents different selectivity, as well preparative HPLC Smartline system (Knauer, Germany) spectrophotometric structures isolated established 1D experiments (Bruker Avance III 400 MHz, Germany). Results discussion. Seven 1–7 ) elucidated. Two 1 2 derivatives ellagic acid, namely 4-O-α-L-arabinofuranoside acid 4-O-β-D-glucopyranoside ), while other five flavonoids: kaempferol-3-O-β-D-glucuronide 3 quercetin-3-O-β-D-glucuronide 4 quercetin-3-O-β-D-(6’’-β-D-glucopyranosyl)-glucopyranoside 5 quercetin-3-O-β-D-(2’’-galloyl)-glucopyranoside 6 (+)-catechin 7 ). Conclusion. As result current research, seven structure Compounds , found L. first time.